摘要
探究影响玉米遗传转化效率的因素,对玉米遗传转化系统的建立与完善有重要意义.以‘宁玉0905’为材料,经农杆菌介导将梭梭的BADH基因转入玉米基因组.结果表明:在黑暗条件下,加入乙酰丁香酮(AS),玉米遗传转化效率提高;当菌液浓度OD600=0.3,浸染时间为5min时,玉米的转化效率最高;农杆菌和愈伤组织共培养2天,玉米的遗传转化效果最好.对转化苗进行PCR检测得出BADH基因已整合到玉米基因组中.当菌液的OD600=0.3,浸染时间为5min,农杆菌和愈伤组织共培养2天时,玉米的转化效率最高.
Abstract
This study explored the effect of factors on Maize transformation efficiency and had important significance to establish and perfect the maize genetic transformation system.Taking the ‘NingYu 0905’ as the study material,the transcription factor BADH gene was transformed into Maize genome of ‘ NingYu 0905’by Agrobacterium tumefaciens.The results revealed that adding acetosyringone could improve the transformation efficiency in corn in darkness.The highest transformation efficiency in corn was obtained when OD600 Agrobacterium tumefaciens was 0.3 for 5 rain.The best transformation efficiency in corn was Agrobacterium tumefaciens and callus in 2 d CO-cultivation.The BADH gene was proved to be transferred into maize genome by PCR test of transformed plants.The highest transformation efficiency in corn was detected in 2 d CO-cultivation after 0.3 of OD600 Agrobacterium tumefaciens for 5 min.
基金项目
宁夏回族自治区科技支撑项目(2012ZYS041)
国家科技期刊平台
NSTL
维普
万方数据