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巨大芽孢杆菌C2产芽孢培养条件的优化

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为了提高巨大芽孢杆菌C2产芽孢的形成率,采用单因素和正交试验,通过摇瓶发酵培养,对影响巨大芽孢杆菌C2芽孢形成的发酵培养基成分和培养条件进行了优化.结果表明:巨大芽孢杆菌C2最优的产芽孢培养基配方为麸皮1.5%、豆粕1.5%、NaCl 0.5%、CaCO3 0.02%、MnSO4·H2O 0.02%、MgSO4·7H2O 0.02%;最适宜培养条件是:培养基初始pH 7.0,接种量O.5%,培养温度37℃,培养时间48 h.在此优化条件下,巨大芽孢杆菌C2芽孢形成率可达到100%,为下一步该茵株在复合生物肥料中的应用打下基础.
Optimization of Spore Culture Conditions for Bacillus megaterium C2
The purpose of this study is to improve the forming rate and the quantity of Bacillus megaterium C2 spores.The medial components and cultural conditions of Bacillus megaterium C2 were optimized by single factor and orthogonal tests in shake-flask fermentation.The results showed that the optimal medial components were composed of 1.5% bran,1.5% soybean meal powder,NaC1 0.5%,0.02% CaCO3,0.02% MnSO4· H2O and 0.02% MgS04· 7H2O.The optimal culture conditions were at temperature 37℃ with initial pH 7.0 and incubation time 48 h.Under these optimum conditions,ormation rate of Bacillus megaterium C2 achieves 100%.The foundation for the application of Bacillus megaterium as a kind of microbial fertilizer was laid.

Bacillus megateriumsporeculture mediumculture conditionsoptimization

王继雯、刘莹莹、李冠杰、刘莉、甄静、巩涛、杨文玲、岳丹丹、慕琦

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河南省微生物工程重点实验室,郑州450008

河南科学院生物研究所有限责任公司,郑州450008

巨大芽孢杆菌 芽孢 培养基 培养条件 优化

2013年河南省基础前沿项目2012年河南省重大科技专项2014年河南省科学院预研项目

132300413219121100110100

2014

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子:0.891
ISSN:1000-6850
年,卷(期):2014.30(36)
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