Cloning and Bioinformatics Analysis of Self-incompatibility Gene SSK1 of Wild Almond in Xinjiang
The research aims to obtain the full-length SSK1 gene with specific expressions in the self-incompatible pollens from wild almond clusters of Xinjiang area.In this research,the leaves and pollen of wild almonds of Xinjiang area were chosen as the materials.Through the rt-PCR and bioinformatics sequencing,the author got a new full-length SSK1 gene,named as PetSSK1.The results showed that the full-length of PetSSK1 was 602 bp and the open reading frame was 534 bp,encoding 177 amino acids.Through the bioinformatics analysis,the molecular formula of PetSSK1 protein was C910H1421N233O295S6 and the relative molecular mass of it was 20538.0,and its estimated half-life was 30 h in theory.Moreover,the result showed that the hydrophobic index and the grand average of hydropathicity of PetSSK1 protein were 41.48 and-0.567,respectively,indicating that PetSSK1 protein belonged to the unstable water-soluble protein.It is the first report that a novel SSK1 gene has been cloned from wild almonds of Xinjiang area.Through the gene cloning and sequence analysis,expression analysis laid the foundation for the future and the effective use of the gene to obtain self-compatible plants.
NETL
NSTL
万方数据
维普
