首页|EMS诱变甘蔗茎尖分生组织的初步研究

EMS诱变甘蔗茎尖分生组织的初步研究

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为探索EMS对离体培养的甘蔗茎尖进行化学诱变的合适浓度和时间组合,以‘新台糖22’和‘粤糖93-159’甘蔗茎尖分生组织为材料,研究不同浓度(24、48、72 μmol/L)、不同时间(2、4h)诱变处理对茎尖分生组织的成活率、鲜重增重量、分化率及分化成苗情况的影响.结果表明:诱变处理后2个基因型的甘蔗茎尖分生组织成活率、每克鲜重增重量、分化率以及成苗的数量和质量都随着EMS处理浓度的升高和处理时间的延长而下降,2个基因型甘蔗茎尖经诱变处理后半分化EMS浓度均小于半致死浓度.综合成活和分化情况,适合‘新台糖22’茎尖分生组织的EMS诱变处理为48 μmol/L处理2h或24 μmol/L处理4h;适合‘粤糖93-159’茎尖分生组织的EMS诱变处理为72μmol/L处理2h或48 μmol/L处理4h.
Mutagenesis Application to Saccharum officinarum Shoot Tip Culture with EMS
In order to find out the optimum dose and time period of ethyl methyl sulphonate (EMS),with which the chemical mutagenesis was applied in sugarcane by treating the stem tip meristem,variations in sugarcane cultivars (‘ROC22’ and ‘YT93-159 ’) were induced through in vitro mutagenesis with varying doses (24,48,72 μmol/L) and time periods (exposed for 2 or 4 h) of EMS.The results showed that the proliferation rate,the percentage of surviving explants,the percentage of shoot tips regenerating shoot,and regenerated shoot development and numbers of the two cultivars declined with the increasing concentration and extending time periods of EMS.Based on some factors such as the percentage of survival and the proliferation rate as well as the reduction of regeneration capacity,48 μmol/L for 2 h or 24 μmol/L for 4 h treatment in ‘ROC22’,and 72 μmol/L for 2 h or 48 μmol/L for 4 h treatment in ‘YT93-159’ were the optimum.

Saccharum officinarumchemical mutagenesisethyl methyl sulphonate (EMS)stem tip meristem

何慧怡、樊丽娜、齐永文、劳方业、邓海华、胡斐

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广州甘蔗糖业研究所/广东省甘蔗改良与生物炼制重点实验室,广州510316

甘蔗 化学诱变 甲基磺酸乙酯 茎尖分生组织

农业部“现代甘蔗产业技术体系高糖聚合育种岗位科学家”广东省科技计划广东省科技计划广东农业厅现代种业工程

CARS-20-1-42012A090300015粤科规财字[2014]208号粤农计[2015]6号

2016

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2016.32(6)
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