Research Progress of Protoplast Culture in Cereal Crops
Plant protoplast has important research value in the application research of variety improvement,genetic transformation and distant hybridization,and in the basic theoretical study of the biology.In order to provide a theoretical basis for the establishment of protoplast culture system and promote the work of the genetic improvement of cereal crops varieties,a detailed elaboration about protoplast culture of cereal crops from four aspects such as protoplast preparation,culture,regeneration and application was made in the article.The main factors affecting the protoplast separation and culture were analyzed,the three ways of protoplast regeneration were summed up,and its applications in genetic improvement and theoretical study were summarized.At the same time,the problems in three aspects including protoplast culture,genetic transformation and cell fusion were put forward and future research directions were also proposed.
NETL
NSTL
万方数据
维普
