Genetic Diversity Analysis of Sugar Beet Resistance to Root Rot by SRAP Markers
The objectives are to identify and analyze sugar beet varieties with good resistance to root rot,and provide a basis for screening and breeding excellent disease-resistant varieties in the future.Field investigation and molecular marker were used in this study.10 pairs SRAP molecule markers were used to investigate the relationship of 11 domestic and foreign varieties.A total of 85 unambiguous bands were obtained by SRAP-PCR,65 of which were polymorphic.The average ratio of polymorphic bands was 76.5%.The average genetic distance was 0.5057,and the genetic similarity among varieties was 0.6031.The results of the field investigation showed that the rate of infection of foreign varieties was 2.9%-4.2%,and the rate of infection of domestic varieties was 2.0%-3.1%.The rate of infection of domestic varieties was generally lower than that of the foreign varieties in this study.11 genotypes were divided into three cluster groups based on cluster analysis (at intercept of 0.2).The results showed that the resistance of domestic varieties to root rot disease was better in this test.'TY301'(domestic),'TY303'(domestic),'CK-TY312'(domestic),and 'HI055'(foreign) had good resistance to root rot.SRAP molecular markers had the reference value for dividing root rot disease resistant varieties of sugar beet.