Molecular Cloning and Bioinformatics Analysis of SFB Genes Controlling Self-incompatibility in Xinjiang Wild Almond (Prunus tenella Batsch.)
The study aims to clone full-length sequence of the new pollen SFB gene of self-incompatibility in Prunus tenella.Xinjiang wild almond was used as the material,and molecular homologous cloning and RT-PCR were conducted in this study.The online software was used to analyze the protein physicochemical properties of SFB.The full-length gene sequences of PtSFB5 and PtSFB6 were successfully cloned.The full-length sequence of the two SFB genes was 1124 bp and 1072 bp,respectively,and encoded 374 aa and 348 aa,respectively.Both of the two genes had F-Box structure,belonged to the F-Box gene family.The predicted relative molecular mass was 30787.26 and 44037.74,and the isoelectric point was 5.93 and 5.43,respectively.PtSFB5 and PtSFB6 protein belonged to the unstable water-soluble protein.
NETL
NSTL
万方数据
维普
