Analysis of Soil Fungal Diversity in Zhaotong Core Tobacco-growing Area Based on High-throughput Sequencing
High-throughput sequencing technology was used to explore the soil fungal diversity from the molecular level in new tobacco area,continuous cropping area with serious soil-borne diseases and typical rotation area in eight tobacco-growing districts in Zhaotong.The results showed that the soil fungal community included 30 phyla,79 classes,202 orders,443 families and 976 genera,and Ascomycota was the absolute dominant phylum,Mortierella and Penicillium were the dominant genera of the soil fungal community.The analysis of community indicator species showed that the relative abundance of the soil fungal community of Ascochyta in new tobacco area was significantly higher than that in continuous cropping area and rotation area.The relative abundance of Purpureocillium,Pseudogymnoascus and Talaromyces in continuous cropping area were significantly higher than that in new tobacco area and rotation area,Fusarium in rotation area was significantly higher than that in new tobacco area,and Mortierella in rotation area was significantly higher than that in continuous cropping area.From the perspective of biodiversity characteristics,soil fungal community in new tobacco area had the highest value,and the species richness was significantly higher than that in continuous cropping area and rotation area.The species richness of soil fungal community in rotation area was significantly higher than that in continuous cropping area,the species evenness was significantly lower than that in continuous cropping area,and the biodiversity of fungal community was slightly higher than that in continuous cropping area.From the perspective of the function of fungal community,the pathotroph,saprotroph,and saprotroph-symbiotroph were main components.In new tobacco area,the top 1 to top 3 of the fungal community were saprotroph-symbiotroph,saprotroph and Pathotroph,that of the continuous cropping area were saprotroph,pathotroph and saprotroph-symbiotroph,and that of the rotation area were saprotroph-symbiotroph,pathotroph-saprotroph-symbiotroph,and saprotroph.The composition of fungal community in tobacco planting soil in Zhaotong was rich.The biodiversity of soil fungal community in new tobacco planting area was the highest,and the species richness was significantly higher than that in continuous cropping area and rotation area.There was no significant difference in the composition of fungal community structure among different types of soil.Rotation was beneficial to reducing the pathotrophic fungal community and the probability of fungal diseases.
万方数据
维普
