EcHPPD Gene of Barnyard Grass:Cloning and Bioinformatics Analysis
In order to clone the gene of hydroxyphenyl pyruvate dioxygenase(HPPD)in Echinochloa crus-galli for exploring its genetic function and better developing herbicide,the homologous cloning and rapid amplification of cDNA ends(RACE)techniques were used to clone the EcHPPD gene sequence using E.crus-galli leaves as materials.The characteristics of EcHPPD were analyzed using bioinformatics software.The coding sequence of EcHPPD gene is 1317 bp in length,encoding a protein consisted of 439 amino acids with a relative molecular weight of 46.659 kD and an isoelectric point of 5.30.E.crus-galli is most evolutionarily close to Dichanthelium oligosanthes(OEL23409.1:8-249)in the Poaceae family based on HPPD protein.The tertiary protein structure of EcHPPD is composed of two chains,with two Fe2+binding sites,and can interact with the pyrazolide active center skeleton through hydrogen bonds.EcHPPD may interact with 11 proteins such as HGO,HPT1,TAT3,AT5G36160 and TAT7.This study reported the EcHPPD gene sequence and conducted bioinformatics analysis,providing a theoretical basis for creating new HPPD inhibitors.
万方数据
维普
