甘薯疮痂病菌的生物学特性和产孢方法研究

Elsinoe batatas Causing Sweetpotato Scab:Biological Characteristics and Conidia Production

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中文摘要：探究甘薯疮痂病菌(Elsinoe batatas)的生物学特性,筛选疮痂病菌在人工培养基上快速、大量地产生分生孢子的方法,为甘薯抗疮痂病育种提供依据.以疮痂病病原菌菌株CRI-CJ2为研究对象,采用菌丝生长速率法测定在不同培养基、pH和光照处理条件下的菌落生长直径,利用凹玻片悬滴法测定在不同温度、pH和光照处理条件下的分生孢子萌发率,比较Fries液体培养法、摇菌法和菌丝涂布法等3种方法的诱导产孢效果.结果表明,最适菌丝生长的培养基为PDA,pH 6～7,致死温度为48℃、10 min,光照对菌丝生长无显著影响,但连续光照使菌落呈鲜红色;分生孢子萌发最适pH 5,最适温度为25℃,致死温度为48℃、10min,光照对分生孢子萌发影响不显著;采用菌丝涂布法可使疮痂病菌在培养第12天分生孢子产孢量达7.09×107个/mL,分生孢子萌发率为24％,产孢量和孢子活力均高于摇菌法,采用Fries液体培养法未观察到分生孢子及产孢结构.可见,选用适宜的培养基、pH能促进菌丝体生长,菌丝涂布法具有产孢快、产孢量大的优点,是一种适合甘薯疮痂病菌分生孢子诱生的方法.

外文摘要：The biological characteristics of the pathogen of sweetpotato scab(Elsinoe batatas)were studied and the methods of producing a large number of conidia in a short time by E.batatas on artificial medium were screened to provide the basis for resistance breeding of sweetpotato scab.Taking the E.batatas isolate CRI-CJ2 as research object,the mycelial growth rate method was utilized to measure the colonial diameter under different medium,pH values and illumination conditions.The concave slide pendent drop method was used to study the conidial germination rate under different temperature,pH values and illumination conditions,and the effects of the Fries liquid culture method,shaking method and mycelium coating method for inducing conidia production of E.batatas were compared.The results showed that the optimal medium for mycelial growth was PDA,the optimum pH value was 6-7,and the lethal temperature was 48℃ for 10 min.No significant differences were found in the mycelial growth between different illuminations,but the colour of colony turned bright red under continuous illumination condition.The optimum pH value for conidial germination was 5,the best temperature was 25℃,and the conidia could not germinate above 48℃ for 10 min.Illumination showed little effect on conidial germination.The conidial quantity of E.batatas was 7.09×107 conidia/mL after 12 days of incubation by the mycelium coating method and the conidial germination rate was 24％after incubation for 24 hours,and the quantity and viability were higher than that by the shaking method.No conidia or sporogenous structure were observed by the Fries liquid culture method.Therefore,selecting appropriate medium and pH value could promote mycelial growth,and the mycelium coating method had the advantages of rapid sporulation and high conidial quantity and was a suitable method for E.batatas conidia induction.

外文关键词：

sweetpotato scabElsinoe batatasbiological characteristicsconidia productionmycelium coating method

作者：

张新新、黄雍、陈景益、邹宏达、罗忠霞、王章英、黄立飞

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作者单位：

广东省农业科学院作物研究所,广东省农作物遗传改良重点实验室,广州 510640

博罗县农业科学研究所,广东惠州 516139

仲恺农业工程学院农业与生物学院,广州 510225

关键词：

甘薯疮痂病 Elsinoe batatas 生物学特性产孢菌丝涂布法

基金：

国家甘薯产业技术体系广东省重点领域研发计划项目广东省甘薯马铃薯产业技术体系创新团队项目广东省驻镇帮镇扶村农村科技特派员项目

项目编号：

CARS-102020B0202190012023KJ111KTP20210313

出版年：

2024

中国农学通报

中国农学会

中国农学通报

CSTPCD

影响因子：0.891

ISSN：1000-6850

年,卷(期)：2024.40(3)

参考文献量10