The contents of toxoflavin(TF)and bongkrekic acid(BA)in foods are determined simultaneously by an ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method.The samples were ultrasonically extracted with 80％methanol aqueous solution.After centrifugation,the supernatants were purified by the QuEChERS EMR-Lipid.And after concentration and reconstitution,the samples were eluted by gradient with 0.1％formic acid solution water and acetonitrile as mobile phases,which was detected by liquid-mass electrospray ion source in positive and negative modes,and quantified by external standard method.The results indicated that the TF and BA showed good linearity in the concentration range of 0.5-100 μg/L,and the correlation coefficient was larger than 0.995.Under different additive concentrations of various substrates,the recoveries were 86.8％-103.6％,and the average relative standard deviations(RSD)were less than 5％.The detection limit of the TF method was 0.1 μg/kg and the quantification limit was 0.2 μg/kg.Meanwhile,the detection limit of the BA method was 0.05 μg/kg,and the quantification limit was 0.1 μg/kg.The method has high sensitivity,good specificity,accuracy and reliability,and is suitable for the determination of TF and BA in foods.
关键词
毒黄素/米酵菌酸/三重四级杆液质法/QuEChERS
Key words
toxoflavin/bongkrekic acid/ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)with triple quadrupole/QuEChER
维普
万方数据