Regulation Effect of Biochar on Bacterial Community in Cotton Field Soil under Saline Water Drip Irrigation
To explore the regulation effects of biochar application on soil physicochemical properties and bacterial communities in cotton field soil under long-term saline water irrigation,based on long-term saline water(8.04 dS/m)irrigation,three treatments were set up for field positioning experiments:no nitrogen fertilizer application(N0),nitrogen fertilizer treatment(N360)and biochar combined with nitrogen fertilizer treatment(BC).Determination of soil bacterial community composition was conducted by high-throughput sequencing technology.The results showed that compared with N0 treatment,N360 treatment significantly increased soil electrical conductivity and total nitrogen,but significantly decreased soil pH and available phosphorus content;BC treatment significantly increased soil water content,electrical conductivity,total carbon,total nitrogen and available potassium content.Compared with N0 treatment,N360 and BC decreased the Shannon and Simpson indices of bacterial communities,while N360 treatment increased the Chao 1 and ACE indices.The dominant bacteria at phylum level were Proteobacteria,Acidobacteriota and Actinobacteriota;the dominant bacteria at genus level were Nitrosospira,RB41 and Sphingomonas.N360 treatment increased the relative abundance of Proteobacteria,Acidobacteriota and Firmicutes,but decreased the relative abundance of Actinobacteriota,Gemmatimonadota and Thermoplasmatota;BC treatment increased the relative abundance of Actinobacteriota,Nitrospirota and Chloroflexi,while decreased the relative abundance of Acidobacteriota,Bacteroidota and Verrucomicrobiota.LEfSe analysis showed that both N360 and BC treatments reduced the number of potential biomarkers of bacteria.The RDA results showed a significant correlation between soil bacterial community structure and soil total nitrogen and available potassium content.Nitrogen fertilizer and Biochar combined with nitrogen fertilizer can regulate soil physicochemical properties,thus regulating soil bacterial community structure,which can form dominant species to adapt to salt environment.
saline water irrigationbiocharnitrogen fertilizercotton field soilbacterial community
万方数据
维普
