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SNP分子标记及其在作物品种鉴定中的应用

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作物品种鉴定是优良品种选育和推广的重要保障,而合适的检测方法是对品种进行准确鉴定的关键.随着分子标记技术的发展,第3代分子标记SNP逐渐应用到品种鉴定领域.本研究概述了 SNP分子标记的特点,分析了高分辨率熔解曲线、竞争性等位基因特异性PCR、基因芯片、测序法、靶向测序基因型检测等5种作物研究中常用的高通量检测方法的特点及适用性,梳理总结了 SNP标记在品种真实性鉴定、纯度检测和亲缘关系分析与分类等方面的研究与应用情况,以期为后续利用SNP分子标记进行品种鉴定提供技术参考.
SNP Molecular Markers:Research and Its Application in Crop Variety Identification
Crop variety identification is an important guarantee for breeding and promoting excellent varieties,and the appropriate detection method is the key for the accurate identification of varieties.With the development of molecular marker techniques,the third-generation molecular marker SNP has gradually been applied in the field of variety identification.This paper outlines the characteristics of SNP molecular markers,analyses the features and applicability of five commonly used high-throughput detection methods in crop research,including high resolution melting,competitive allele specific PCR,gene chips,sequencing and genotyping by target sequencing.The research and application of SNP markers in the identification of variety authenticity,purity testing,and analysis of genetic relationships and classification are summarized as well,in order to provide technical reference for subsequent variety identification.

SNP markshigh-throughput genotypingauthenticity identificationpurity testgenetic relationship analysis

田海燕、张海娜、王永强、周永萍、张莹璐

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河北省农林科学院棉花研究所/农业农村部黄淮海半干旱区棉花生物学与遗传育种重点实验室,国家棉花改良中心河北分中心,石家庄 050051

SNP标记 高通量基因分型 真实性鉴定 纯度检测 亲缘关系分析

河北省农林科学院科技创新专项课题河北省农林科学院棉花研究所所立项目

2022KJCXZX-MHS-9

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子:0.891
ISSN:1000-6850
年,卷(期):2024.40(6)
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