首页|基于ISSR标记分析连云港地区野生灵芝种质资源遗传多样性及亲缘关系

基于ISSR标记分析连云港地区野生灵芝种质资源遗传多样性及亲缘关系

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为分析连云港地区野生灵芝种质资源之间的亲缘关系和多样性水平,以连云港地区15株野生灵芝为研究对象,采用ISSR分子标记进行遗传多样性及亲缘关系分析.15株野生灵芝中扩增出多态性条带为40条,片段大小在250～2000 bp之间,平均等位基因位点1.8个,平均有效等位基因数1.2454,平均Nei's遗传多样性0.1742,平均Shannon信息指数0.2925,有效等位基因数(Ne)的变化范围为1.0000～1.6423,Nei's遗传多样性(He)的变化范围为0.1031～0.3911,Shannon信息指数(I)的变化范围为0.2235～0.5799,根据ISSR分子标记的遗传聚类图,15株灵芝相似系数范围在0.415～0.866之间,相似系数为0.63时,可以将15株野生灵芝分为四组.研究结果为连云港地区灵芝种质资源分类、保护和品种创制提供了参考依据.
Wild Ganoderma lingzhi Resources in Lianyungang Area:Genetic Diversity and Genetic Relationship Analysis Based on ISSR Markers
In order to analyze the genetic relationships and diversity levels of wild Ganoderma lingzhi germplasm resources in Lianyungang area,the genetic diversity and genetic relationship of 15 wild Ganoderma lingzhi in Lianyungang area were analyzed by ISSR molecular markers.The results showed that 40 polymorphic bands were amplified,the fragment size ranging from 250 to 2000 bp,the average allele number was 1.8,the average effective allele number was 1.2454,the average Nei's genetic diversity was 0.1742,and the average Shannon information index was 0.2925.The effective allele number(Ne)ranged from 1.0000 to 1.6423,the genetic diversity of Nei's(He)ranged from 0.1031 to 0.3911,and the Shannon Information index(I)ranged from 0.2235 to 0.5799.According to the genetic clustering diagram of ISSR molecular markers,the similarity coefficient of 15 Ganoderma lingzhi strains ranged from 0.415 to 0.866.When the similarity coefficient is 0.63,the 15 wild Ganoderma lingzhi strains could be divided into four groups.The results provided a reference for the classification,protection and variety creation of Ganoderma lingzhi germplasm resources in Lianyungang area.

Ganoderma lingzhiinter-simple sequence repeatgenetic diversitycluster analysisgenetic relationship

纪伟、苏文英、刘晓梅、王一璞、任立凯、奚小艳、陈克龙

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连云港市农业科学院,江苏连云港 222000

青海师范大学,西宁 810000

灵芝 简单序列重复区间 遗传多样性 聚类分析 亲缘关系

江苏现代农业产业技术体系"江苏现代农业产业技术体系连云港食用菌推广示范基地"连云港市财政专项省级现代农业发展连云港市食用菌全产业链建设项目连云港市科协软课题项目

JATS[2022]177QNJJ2209连农复202314号Lkxyb23027

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2024.40(6)
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