Polyphenol Metabolites During Flue-curing in Tobacco'Cuibi NO.1'Leaves:Dynamic Changes Based on Metabolomics Analysis
In order to reveal dynamic changes of polyphenol metabolites that influence the modulation characteristics and aroma quality of tobacco leaves during curing process,using the upper leaves of tobacco variety of'Cuibi NO.1'(CB-1)as the material,a total of 15 samples during curing process were collected and analyzed by UPLC-MS/MS.Cluster analysis of the differential metabolites was conducted based on K-means,and the metabolites annotation and metabolic pathway analysis were conducted based on KEGG database.A total of 1069 metabolites were identified from 15 samples,including flavonoids,phenolic acids,lipids,alkaloids,amino acids etc.Notably,374 of polyphenols were identified,accounting for 34.99％of the total metabolites.The differential metabolites and their K-means cluster analysis showed that most significant changes in metabolite content occurred during the transition from the yellowing stage to the early color-fixing stage.Furthermore,these metabolites were found to be enriched in pathways such as tyrosine metabolism,phenylalanine metabolism,phenylpropanoid biosynthesis,and flavonoid biosynthesis.Additionally,the differential metabolites were found to be enriched in the biosynthesis pathways of hydroquinone,chlorogenic acid,kaempferol,and sinapic acid,which were closely related to tobacco flavor and quality.The dynamic changes of polyphenol metabolites during the tobacco leaf flue-curing process were illuminated based on the metabolomics,and these findings lay a foundation for understanding the material basis of tobacco quality formation and the regulation of tobacco browning.
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