Effects of Exogenous Addition of Benzoic Acid on Seed Germination and Seedling Growth of Perilla frutescens
To investigate the effects of exogenous benzoic acid stress on seed germination,seedling growth and antioxidant enzyme activity of perilla seeds,perilla seeds petri dish filter paper culture and indoor potting test were conducted by adding benzoic acid,and six gradients were set:0(CK),1,1.5,2,2.5 and 3 mmol/L.The effects of different concentrations of benzoic acid stress on germination percentage,germination energy,germination index,viability index,perilla seedling growth and antioxidant enzyme activities were studied.The results showed that with the increase of benzoic acid concentration,the germination rate,germination potential,germination index and viability index of perilla seeds were generally promoted by low concentration and inhibited by high concentration.The root weight,radicle length,cotyl length and plant fresh weight of perilla seedlings showed a significant downward trend,and the activities of catalase(CAT)and peroxidase(POD)in seedling leaves showed a significant decreasing trend(P＜0.05),the activity of superoxide dismutase(SOD)first increased and then decreased,and reached maximum at 1.5 mmol/L.The effect of exogenous addition of benzoic acid on the germination of perilla seeds is related to the concentration of benzoic acid(low concentration promotion and high concentration inhibition),which has an inhibitory effect on the growth of perilla seedlings,confirming the self-toxicity effect of benzoic acid on the growth of perilla.
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