Physiological Response and Cold Resistance Evaluation of the Shoots of Gleditsia sinensis under Low Temperature Stress
This study aims to assess the cold resistance of the shoots of G.sinensis by analyzing the difference of cold resistance among its different clones,providing theoretical basis for the cultivation and breeding of cold resistant varieties of G.sinensis.One-year-old shoots of 11 clones of G.sinensis were selected as experimental materials,the relative electrical conductivity(REC)of them under different low temperature conditions(5 ℃,0 ℃,-5 ℃,-10 ℃,-15 ℃,-20 ℃,-25 ℃)was measured and analyzed,respectively.Principal component analysis and cluster analysis were used based on the results of REC.The anatomical structure of shoots,reflection spectrum,contents of malondialdehyde(MDA)and activities of super oxide dismutase(SOD)were studied under low temperature stress to analyze the difference of cold resistance among the clones.The results showed that there were some differences in the variations of REC in the shoots of all clones under the low temperature stress.There were some characteristics in clones with a stronger cold resistance,including a larger ratio of xylem,a smaller ratio of cortex and phloem in the cross-sectional area of branches,and higher values of NDVI,ARI2,SIPI and CRI2 parameters.At the same time,these clones presented the characteristics of low MDA contents and high SOD activities.The cold resistance of the 11 shoots of G.sinensis was in order of'Yangjiaozao'＞'LKHD5'＞'Beishandi'＞'Shuaijia 3 hao'＞'Niujiayu'＞'302'＞'Helin 1 hao'＞'Jiantoucun'＞'Xijia 2 hao'＞'LKHD4'＞'LKSD'.
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