Identification of Actinomyces 4-1 and Study on Bacteriostasis and Stability of Its Fermentation Broth
In order to explore the bacteria resources of saline-alkali soil and test their antibacterial function,actinomycete 4-1 was isolated and used as the original strain.Combined with morphological characteristics,physiological and biochemical indicators,as well as molecular biology techniques,the species status of 4-1 was comprehensively identified,while the antibacterial activity and stability of its fermentation broth were evaluated using agar diffusion method and mycelial growth rate inhibition method.The results showed that strain 4-1 showed the highest similarity to Streptomyces puniceus,with a homology of 99.93％,and the constructed phylogenetic tree showed that they were most closely related.The fermentation broth of strain 4-1 had a wide range of antibacterial spectrum and showed certain inhibitory effects on pathogenic bacteria(6 strains)and pathogenic fungi(5 strains).The stability of the fermentation broth was minimally affected by acidity,alkalinity,heat,or ultraviolet radiation.However,the antibacterial activity of the fermentation broth decreased when the temperature exceeded 90℃ or the pH was below 4.The availability and functional evaluation of this strain laid a foundation for further expanding rhizosphere growth-promoting bacteria resources and applications.
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