首页|甜菜酪氨酸代谢途径关键酶的生物信息学研究

甜菜酪氨酸代谢途径关键酶的生物信息学研究

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为了了解甜菜中酪氨酸代谢途径关键酶的特性和进化关系,以甜菜、菠菜、大豆等植物为试验材料,利用NCBI数据库收集了酪氨酸代谢途径5种关键酶(PPO、TAT、TYDC、HPPD、HPPR)的序列信息.使用ExPASy-ProtParam软件和MEGA-7软件分析了关键酶的理化性质,同时将它们序列间的相似性及演化距离进行对比,并且构建系统发育树.理化性质分析结果显示,甜菜酪氨酸代谢途径中5种关键酶均为不稳定的亲水性蛋白质,甜菜PPO的氨基酸数量最高;系统发育树分析结果显示,甜菜与菠菜的PPO和HPPD相似性高;甜菜分别与大豆的TAT、马铃薯的TYDC、大麦的HPPR相似性高.该研究为参考其他作物探索甜菜酪氨酸代谢途径关键酶功能研究提供参考,为进一步研究甜菜基因组学奠定基础.
A Bioinformatics Study on Key Enzymes Involved in Tyrosine Metabolism in Beet
To understand the characteristics and evolutionary relationships of key enzymes involved in the tyrosine metabolism pathway in sugar beets,taking plants such as sugar beet,spinach,and soybean as experimental materials,sequence information of five key enzymes in the tyrosine metabolism pathway(PPO,TAT,TYDC,HPPD,HPPR)was collected using the NCBI database.The physicochemical properties of the key enzymes were analyzed using ExPASy-ProtParam software and MEGA-7 software,their sequence similarities and evolutionary distances were compared,and a phylogenetic tree was constructed.The analysis of physicochemical properties showed that all five key enzymes in the tyrosine metabolism pathway of sugar beet were unstable hydrophilic proteins,and the number of amino acids in sugar beet PPO was the highest;the phylogenetic tree analysis results showed that there was high similarity in PPO and HPPD between sugar beet and spinach;beets had high similarity with TAT of soybeans,TYDC of potatoes,and HPPR of barley,respectively.This study provided a reference for other crops to explore the key enzyme function of sugar beet tyrosine metabolism pathway and laid a foundation for further research on sugar beet genomics.

beettyrosinecatalytic enzymemetabolic pathwaybioinformatics analysis

杨家琪、韩广源、刘乃新、周芹

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黑龙江大学现代农业与生态环境学院,哈尔滨 150080

哈尔滨海关技术中心,哈尔滨 150023

甜菜 酪氨酸 催化酶 代谢途径 生物信息学分析

国家糖料产业技术体系建设项目黑龙江省博士后基金

CARS-170503LBH-Z22245

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子:0.891
ISSN:1000-6850
年,卷(期):2024.40(9)
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