Antibacterial Activities of Litter Fungus Neocucurbitaria salicis-albae and Its Metabolite
This study focuses on discovering the antimicrobial fungal resources from forest litter fungi,screening out and identifying the fungal strains with antimicrobial activities,and isolating and purifying their antimicrobial compounds.The forest litter from the Greater Hinggan Mountains was selected as test materials,then the fungi were isolated by particle separation method,the taxonomic data were measured by gene sequence comparison,the antibacterial activity was measured by well diffusion method,the monomeric compounds were isolated by column chromatography,and the chemical structure was determined by spectral analysis.Fungal strain SGSF801 was isolated and identified as a new strain of Neocucurbitaria salicis-albae by multiple sequence analysis.The results of the antimicrobial assay showed that the rice fermentation products had strong antimicrobial activities against plant pathogens Ralstonia solanacearum,Pseudomonas syringae and Rhizoctonia solani.The product of fungal rice fermentation was isolated and purified to obtain a monomeric compound,which was identified as Cercosporamide by 1H-NMR and 13C-NMR,and its molecular formula was C16H13NO7.Its MIC values were 31.25-62.5 µg/mL for Ralstonia solanacearum and 125-250 µg/mL for Pseudomonas syringae.In this study,Cercosporamide was isolated from fungal genus Neocucurbitaria for the first time,and it was found to have antibacterial activities against plant pathogens,which provided a basis for further utilization of litter fungi resources.
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