首页|植物乳杆菌HDL-03胞外多糖合成条件的优化研究

植物乳杆菌HDL-03胞外多糖合成条件的优化研究

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为提高乳酸菌胞外多糖(EPS)产量，促进其开发及应用，本研究以植物乳杆菌(Lactiplantibacillus plantarum)HDL-03为出发菌株，通过单因素试验优化菌株产EPS的发酵条件，提高EPS产量。结果表明，L。plantarum HDL-03的最佳产EPS发酵条件为:蔗糖70 g/L、蛋白胨6 g/L、牛肉膏8 g/L、酵母浸粉7 g/L、乙酸钠1 g/L、硫酸镁0。3 g/L、磷酸氢二钾1 g/L、柠檬酸铵3 g/L、初始pH 6。5、培养温度30℃、摇床转速120 r/min、接种量3％，优化后L。plantarum HDL-03的EPS终产量为80。0 g/L，相比于优化前的EPS产量提高了 2。3倍。本研究提高了乳酸菌EPS的产量，有助于其分离纯化及工业化生产。
Exopolysaccharides from Lactiplantibacillus plantarum HDL-03:Synthesis Conditions Optimization
In order to improve the yield of exopolysaccharide(EPS)of lactic acid bacteria and promote its development and application,Lactiplantibacillus plantarum HDL-03 was used as the test strain and the fermentation conditions of the EPS were optimized by single factor experiment to improve production.The results indicated that the optimal fermentation conditions of L.plantarum HDL-03 were sucrose 70 g/L,peptone 6 g/L,beef paste 8 g/L,yeast extract 7 g/L,sodium acetate 1 g/L,magnesium sulfate 0.3 g/L,dipotassium hydrogen phosphate 1 g/L,ammonium citrate 3 g/L and initial pH 6.5.Culture temperature was 30℃,shaking table rotation speed was 120 r/min and inoculum size was 3％.The final EPS yield of L.plantarum HDL-03 after optimization was 80.0 g/L,which was 2.3 times higher than the EPS yield before optimization.In this study,the yield of EPS produced by lactic acid bacteria was increased,which contributed to its separation,purification and industrial production.

Lactiplantibacillus plantarumexopolysaccharidesingle factorfermentation conditionsoptimization

周渤森、曹慧莹、齐心彤、于连升、杨义、葛菁萍、宋刚、杜仁鹏

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黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,哈尔滨 150080

河北环境工程学院,河北省农业生态安全重点实验室,河北秦皇岛 066102

植物乳杆菌 胞外多糖 单因素 发酵条件 优化

黑龙江省自然科学基金优秀青年基金项目中国博士后科学基金资助项目黑龙江省博士后资助项目新时代龙江优秀硕士、博士学位论文项目河北省农业生态安全重点实验室开放基金课题项目黑龙江省省属高等学校基本科研业务费科研项目

YQ2021C0302022MD713755LBH-Z21082LJYXL2022-0202023SYSJJ172022-KYYWF-1075

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2024.40(11)
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