Technology of Improved CT AB Method for Extracting Total RNA from Different Crops
In order to improve the operation efficiency,a simple and effective method for crop total RNA extraction was developed.Total RNA was extracted from different tissues of rape,cotton,peanut,wheat,potato,corn,soybean,zucchini and other crops by CTAB method,improved CTAB method and TanSzol Reagent kit method.The detection results showed that the quality of total RNA extracted by improved CTAB method was higher.High total RNA yields were obtained in rape petals,wheat,peanut and zucchini leaves,which were 24.5,23.7,26.4 and 17.35 μg/mL,respectively;and the OD260/280 and OD260/230 values were 1.90,1.73,1.72,1.80 and 2.10,1.50,2.01,1.70,respectively.By comprehensive comparison of the above three methods,the improved CTAB method is suitable for total RNA extraction of rape,wheat,peanut,zucchini and other crops,which lays a foundation for subsequent molecular biological detection.
万方数据
维普
