不同波长LEDs光源对苦荞芽菜生长、生物活性物积累及抗氧化活性的影响
Effects on Growth,Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts:Different Wavelengths of LEDs Light
陈丽丽 1吕平 2吕少杰 2房明志 2李斌 2胡跃高 3薛绪掌 4康文艺5
作者信息
- 1. 河南大学农学院,河南开封 475000;浙江道济农业科技发展有限公司,浙江嘉兴 314504;中国农业大学农学院,北京 100193;宁波大学科学技术学院,浙江宁波 315300
- 2. 浙江道济农业科技发展有限公司,浙江嘉兴 314504
- 3. 中国农业大学农学院,北京 100193
- 4. 北京市农林科学院,农业智能装备研究中心,北京 100097
- 5. 河南大学农学院,河南开封 475000
- 折叠
摘要
为探究不同波长LEDs光源对苦荞芽菜生长的影响,本研究以黑暗(D)作对照,采用单色红(R)、蓝(B)、绿(G)光源培养苦荞芽菜,收获后分别测定其形态指标、生物活性物积累量、类黄酮合成关键基因表达量及抗氧化活性指标,结果表明:B处理的苦荞芽菜下胚轴呈深红色,伸长生长受到强烈抑制,花青素、总黄酮及总酚积累量均最高,分别是D的7.18、2.96和2.49倍;R处理的下胚轴呈浅粉色,上述3种生物活性物积累量分别是对照的2.56、1.68和1.40倍;G处理的下胚轴伸长与R相似,外观呈透明白色,生物活性物积累量与对照无显著差异.B和R均强烈诱导类黄酮生物合成通路上关键结构基因表达,显著增加苦荞芽菜下胚轴抗氧化活性.此外,各单色光处理均未显著影响苦荞芽菜可食用部位的鲜重.由此可见,红、蓝LEDs光源可显著影响苦荞芽菜形态生长,并增加其生物活性物积累及抗氧化能力,以蓝光最佳.
Abstract
In order to investigate the effects of different wavelengths of LEDs light sources on the growth of tartary buckwheat sprouts,red(R),blue(B)and green(G)LEDs were used to culture the sprouts,with the darkness as a control.The indicators including morphological growth,bioactive matter accumulation,key gene expression involved in flavonoid synthesis,and antioxidant activity were determined,respectively.The results showed that the hypocotyls of tartary buckwheat sprouts under B were deep red and the hypocotyl elongation was strongly inhibited.The accumulation of anthocyanins,total flavonoids and total phenols under B were the highest,which were 7.18,2.96 and 2.49 times as that of D,respectively.The hypocotyl of tartary buckwheat sprouts under R showed a light pink color.The accumulation of the above three bioactive substances under R was also significantly higher than that of the control,which was 2.56,1.68 and 1.40 times,respectively.The hypocotyl elongation of the sprouts under G was similar to that of R,but the appearance was transparent and white.The accumulation of bioactive matters under G was not significantly different from that of the control.Both B and R strongly induced the expression of key genes involved in flavonoid biosynthesis pathway,and significantly increased the antioxidant activity of the hypocotyls of tartary buckwheat sprouts.In conclusion,red and blue LEDs could significantly affect the morphological growth of tartary buckwheat sprouts,and increase the accumulation of bioactive substances as well as the antioxidant capacity.Meanwhile B treatment was recommended due to the best performance.
关键词
红/蓝/绿LEDs光源/苦荞芽菜/生物活性物/抗氧化活性/类黄酮合成基因表达Key words
red/blue/green LEDs light source/tartary buckwheat sprouts/bioactive substance/antioxidant activity/flavonoid synthesis gene expression引用本文复制引用
基金项目
国家农业产业技术体系燕荞麦东北栽培岗(2016AA103005)
宁波大学科学技术学院高层次人才科研启动经费(RC100009)
出版年
2024
国家科技期刊平台
NSTL
万方数据