The Pathogen Causing Leaf and Stem Spot of Houttuynia cordata:Isolation and Identification
To identify the pathogen species causing a noval desease of leaf spot and stem spot of Houttuynia cordata Thunb.in Guanghan City,Sichuan Province,and provides theoretical basis for the scientific management of this disease,the leaves and stems of diseased H.cordata were collected from Guanghan City,and the pathogen was isolated and identified by tissue isolation,pathogenicity testing,morphological characterization,and molecular identification based on ITS,tef1,cal and his3 gene sequences.The results indicate that the mycelium of the pathogen is gray-white to light brown.The conidiomata,which are produced in the late growth stage,can produce two types of conidia:fusiform to oval-shaped a-type conidia and filamentous or hook-shaped β-type conidia.Multiple gene cluster analysis showed that the pathogen formed an individual branch adjacent to the Diaporthe Sojae and D.phaseolorum branches,and formed a big branch with these two branches in the phylogenetic tree,and a new species named D.houttuynia was suggested to establish to accommodate this pathogen.According to morphology and molecular identification,the pathogen causing the noval desease of leaf spot and stem spot of H.cordata in Guanghan City was identified as D.houttuynia.
Houttuynia cordataleaf spotstem spotisolation and identificationDiaporthe houttuynia
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