Survey and Pathgen Identification of Rice Root Knot Nematode Disease in Rice-Vegetable Rotation Fields in Guangxi
In order to investigate the occurrence of root knot nematode disease in rice-vegetable rotation fields of Guangxi,and clarify the pathogen species,a sampling survey on the occurrence of rice root-knot nematode disease was carried out in 171 rice-vegetable rotation fields in 27 townships of 14 cities in Guangxi.Nematodes were isolated from samples,and the species was identified by observing the morphological characteristics of second instar larvae and perineal pattern of females,and analyzing ribosomal ITS region and 28S rDNA D2D3 region sequences.The results showed that 64 fields of them were affected with root-knot nematodes,with a detection rate of 37.4％during the rice-growing season and 25.7％during the vegetable rotation.The species of root-knot nematode on rice in Guangxi was confirmed as Meloidogyne graminicola.It indicated that the disease caused by M.graminicola had occurred widely in rice-vegetable rotation fields in Guangxi,and more attention should be paid.
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