This study aimed to analyze the free amino acid components of wild soybean resources which were collected from various regions of Heilongjiang Province.17 kinds of free amino acid content in 153 wild soybean resources were determined and analyzed systematically.The results indicated that the amplitude variation of methionine content was the highest among the 17 kinds of free amino acids with a variation coefficient of 0.469.The total free amino acid content was 2.069 mg/g,among which the arginine content was the highest(0.894 mg/g).Positive correlation was showed between any free amino acids and total free amino acids.There were significant positive correlations among most amino acids,while significant negative correlation could be found between aspartic acid and phenylalanine and between serine and arginine.The cumulative contribution rate of the first five principal components extracted from the principal component analysis reached 85.15%.The contribution rates of the first and second principal components were 26.47%and 20.55%,respectively.According to the first and second principal components,large discrepancies were discovered between the resources in the northern region and the central and southern regions,and small discrepancies were discovered between the resources in the eastern and western regions.In this study,the content variation and correlation among components of 17 kinds of free amino acids in wild soybean in cold region were analyzed,and the effects of different sources on the content and components of free amino acids were clarified,which provided important basic data for soybean breeding applications.