Screening,Identification and Control Effect of Biocontrol Bacteria Against Kiwifruit Black Spot Disease
In order to screen the antagonistic bacteria against Diaporthe eres,strain BKH with good antagonistic effect was isolated from the rhizosphere soil of Actinidia chinensis in Zhouzhi County,Shaanxi Province by plate confrontation method.BKH strain was identified as Burkholderia ambifaria,through the methods of colony and microscopic morphology observation,physiological and biochemical test and 16S rDNA gene sequences analysis.BKH strain could significantly inhibit the growth of Diaporthe eres on the plate,and the inhibition rate was 72.5%.The field control effect of BKH strain against kiwifruit black spot disease was 91.7%,which had great potential in biological control of kiwifruit black spot disease.
kiwifruit black spot diseaseBurkholderia ambifariabiocontrol bacteriaidentificationcontrol effect