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猕猴桃黑头病生防菌的筛选、鉴定及防效评价

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研究旨在筛选对猕猴桃黑头病病原真菌(Diaporthe eres)有拮抗作用的菌株。通过平板对峙法从陕西周至县猕猴桃根际土壤中分离筛选到1株具有明显拮抗作用的菌株BKH。显微及菌落形态观察、生理生化试验和16S rDNA序列分析结果表明,该菌株为双向伯克霍尔德氏菌(Burkholderia ambifaria)。菌株BKH在平板上能显著抑制Diaporthe eres生长,抑菌率为72。5%,且对猕猴桃黑头病田间防效可达91。7%,在猕猴桃黑头病生物防治方面具有应用潜力。
Screening,Identification and Control Effect of Biocontrol Bacteria Against Kiwifruit Black Spot Disease
In order to screen the antagonistic bacteria against Diaporthe eres,strain BKH with good antagonistic effect was isolated from the rhizosphere soil of Actinidia chinensis in Zhouzhi County,Shaanxi Province by plate confrontation method.BKH strain was identified as Burkholderia ambifaria,through the methods of colony and microscopic morphology observation,physiological and biochemical test and 16S rDNA gene sequences analysis.BKH strain could significantly inhibit the growth of Diaporthe eres on the plate,and the inhibition rate was 72.5%.The field control effect of BKH strain against kiwifruit black spot disease was 91.7%,which had great potential in biological control of kiwifruit black spot disease.

kiwifruit black spot diseaseBurkholderia ambifariabiocontrol bacteriaidentificationcontrol effect

王家哲、张锋、李英梅、周搏航、付博

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陕西省生物农业研究所,西安 710043

猕猴桃黑头病 双向伯克霍尔德氏菌 生防菌 鉴定 防效评价

西安市科技局科技项目西安市科技局农业重点产业链关键技术攻关项目

21NYYF002622NYGG0007

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子:0.891
ISSN:1000-6850
年,卷(期):2024.40(13)