Amomun tsao-ko Germplasm Resources in Different Origins:Cold Resistance Evaluation
The research aims to screen out the germplasm resource of Amomun tsao-ko with high cold resistance,provide a basis for hybrid breeding and cultivation of Amomun tsao-ko and facilitate the sustainable development of the Amomun tsao-ko industry.Taking Amomun tsao-ko plants from different origins which has been grown for 6 months as test materials,the materials were cultured under low temperature stress for 3 days(low temperature incubator parameters:4℃,12 hours of darkness,12 hours of light,90%humidity),the physiological indexes related to cold resistance were measured,and cold resistance of the Amomun tsao-ko germplasm resources of different origins was comprehensively evaluated by using correlation anailsis,principal component analysis and membership function analysis.The results showed that under low temperature(4℃)stress,the chlorophyll content and antioxidant enzyme activity in the leaves of Amomun tsao-ko decreased,while the electrical conductivity,malondialdehyde content,soluble sugar content,and soluble protein content increased to varying degrees.The rank of the cold resistance of the Amomun tsao-ko obtained by the membership function analysis is DH-LC>BS-TC>NJ-GS-GLB>WS-MG>PE-ZY>PE-LC.Among the 6 germplasms from different origins,DH-LC has the best cold resistance,and it could be used as cold resistance material for hybrid breeding and cultivation.
Amomun tsao-kogermplasm resourceslow temperature stresscold tolerance screeningmembership function analysis
NETL
NSTL
万方数据
