Effects of Polyvinylpyrrolidone on Callus Browning and Antioxidant Activity of Medicago sativa cv.'Gannong No.3'
In order to investigate the effect of different concentrations of Polyvinylpyrrolidone(PVP)on browning of alfalfa callus,hypocotyls of alfalfa'Gannong No.3'were selected as explants to study the inhibitory effect of different concentrations of PVP on browning of alfalfa callus.The results showed that different concentrations of PVP had different effects on callus browning.Compared with control,browning rate of treatment groups decreased.When PVP concentration was 300 mg/L,browning degree decreased significantly(P<0.05).The MDA content and PPO activity reached the lowest of 0.1 μmol/g FW and 32.8 U/g respectively.POD activity was lower(232.0 U/g),SOD activity was higher(168.1 U/g).Therefore,PVP 300 mg/L had the best inhibitory effect on browning of alfalfa callus.
NETL
NSTL
万方数据
