Genome Survey Analysis of Curculio chinensis(Coleoptera:Curculionidae)
The camellia weevil,Curculio chinensis Chevrolat,is a host-specific predator of the seeds of oil-tea camellia,endemic woody oil-bearing plant in China.It is one of the dangerous pests in Chinese forestry,and is widely distributed in different oil-tea camellia planting areas of China with increasing damage.In order to further study its genetic basis and host adaptability,and determine sequencing strategies that were suitable for genome sequencing of Curculionidae in Coleoptera,survey sequencing was first adopted to lay the foundation for further deep genome sequencing and large-scale population sequencing.In this study,reads from second-generation high-throughput sequencing technology(MGISEQ-2000 Pair-End sequencing platform)was used to determine the genome size of C.chinensis,and estimate the heterozygosity,repetition rate and GC content of the genome mass of this species.The results showed that the genome size of C.chinensis was about 1356.82 Mb,and the sequencing depth reached 50×.K-mer analysis showed that although there was a heterozygous peak in the C.chinensis genome,the heterozygous rate was as low as 1.20％.And the content of repeated sequences was about 77％.The results of this study are of great significance to reveal the adaptive evolution of Camellia weevil,and can provide a basis for the selection of strategies for the subsequent construction of a complete genome and multi-population genome sequencing.
NETL
NSTL
万方数据
