Improving Broccoli Soil Nutrients and Fungal Community:Application of Different Rotation Patterns in Soil Health
To solve the soil health problems such as fertility decline and quality degradation caused by unreasonable factors in the utilization and management of cultivated land resources,a field experiment was conducted to study the effects of planting broccoli under different rotation patterns on soil nutrients,fungal community structure and diversity.The rhizosphere soil of broccoli after different rotation modes was used as the research object,and the soil chemical properties and enzyme activities were measured and analyzed.Six rotation modes were set,including continuous cropping of broccoli(BB),broccoli-millet(MB),broccoli-onion(OB),broccoli-soybean(SB),broccoli-potato(PB)and broccoli-welsh onion(GB),with broccoli as a control.Illumina MiSeq high-throughput sequencing technology was used to analyze the fungal community structure and diversity under different rotation modes.The results showed that compared with the continuous cropping of broccoli,the chemical properties of soil rhizosphere in each rotation treatment were significantly different,and there was no significant difference in pH,and the chemical properties of MB were significantly increased.By Alpha diversity analysis,the Chao1 index and the observed species number index of other treatments showed an upward trend compared with BB,while the Simpson index showed a downward trend.As for the Shannon index and Pielou's evenness index,the performance was SB＜GB＜MB＜BB＜PB＜OB.The results of the abundance and composition of the fungal community showed that the dominant flora was composed of chytridiomycota,ascomycota,sporophytes and chaetomium.Correlation analysis showed that total phosphorus and nitrate nitrogen were the main factors affecting soil beneficial bacteria.By comprehensive comparison,MB can significantly optimize soil chemical properties,enhance enzyme activity,and increase the abundance and diversity of beneficial microbial communities,thereby enhancing soil nutrient regulation and plant disease resistance.MB is the best in all aspects of indicators,which can effectively alleviate the continuous cropping of broccoli and improve soil health problems.It is recommended as a promotion planting mode.
broccolirotationsoil chemical propertiessoil microorganismssoil health
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