Essential Oil Preparation and Volatile Components Analysis in Acanthopanax senticosus Leaves
In order to compare the yield of essential oil from Acanthopanax senticosus leaves prepared by different extraction methods and to analyze its quality,Acanthopanax senticosus leaves were used as raw materials,essential oils were prepared using both steam distillation and supercritical CO2 extraction methods,followed by the analysis of volatile components using GC-MS.Additionally,the prepared oils were analyzed for their antibacterial and antioxidant activities.The yield of essential oil obtained by steam distillation was 0.10％,whereas the yield obtained by supercritical CO2 extraction was 0.47％.GC-MS analysis identified 35 components out of 57 separated from the essential oil prepared by steam distillation,and 57 components out of 94 separated from the oil prepared by supercritical CO2 extraction.Antibacterial tests indicated that Acanthopanax senticosus leaf essential oil possessed certain antibacterial activity.Moreover,the essential oil exhibited good antioxidant activity,with a DPPH radical scavenging rate of 30.7％and an IC50 value of 3.510±0.175 μg/mL.The results suggest that Acanthopanax senticosus leaf essential oil contains a variety of aromatic compounds that not only contribute to the oil's fragrance but also possess multiple active properties,providing a reference for the rational use of Acanthopanax senticosus leaves and the preparation of essential oils.
Acanthopanax senticosus leavessupercritical CO2 extractionessential oilGC-MSsteam distillationantibacterial activityantioxidant activity
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