Detection and Survival Time of Erwinia amylovora in Mature Korla Fragrant Pear
To determine whether the Erwinia amylovora was on the mature pear and its survival time,mature fruits from different pear orchards in Korla region were collected.Through the quantitative detection method of living bacteria,the dynamic detection of E.amylovora on the stored pear was carried out,and the pathogenicity of the strains isolated from Zeller selective medium was determined.The survival time of E.amylovora in different parts of pear was as follows:surface(133 d)＞calyx(83 d)/stalkhole(83 d)＞peduncle(63 d).The viable cells of E.amylovora detection rates of different parts were as follows:fruit surface(6.88％)＞calyx(3.75％)＞stalkhole(2.19％)＞peduncle(1.57％),and the isolated strains of E.amylovora had pathogenicity.In summary,strengthening the quarantine of Korla fragrant pear is an important method to prevent the disease from spreading across regions,and the results provide a theoretical basis for the risk assessment of pear as the vector of E.amylovora.
pearfire blightErwinia amylovoradynamic detectionsurvival time
NETL
NSTL
万方数据
