首页|不同栽培模式下人参根际土壤代谢物的差异研究

不同栽培模式下人参根际土壤代谢物的差异研究

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本研究旨在比较仿野生林下栽培人参(PSM)与农田栽培人参(NPSM)根际土壤代谢物的差异,以期为提升林下栽培人参的品质提供科学依据。通过采用气相色谱-串联质谱(GC-MS/MS)检测技术和分子生物学方法,分析了仿野生林下栽培人参和农田栽培人参根际土壤代谢物的差异。结果显示,仿野生林下栽培人参和农田栽培人参根际土壤代谢物具有差异,在检测到的68种差异代谢物中,有17种代谢物表现出显著差异,其中14种上调,3种下调。这些差异代谢物主要包括糖类、脂质类、有机酸类、酯类等物质。其中糖类占总显著差异代谢物的47%,是差异代谢物的主要部分;这些根际土壤差异代谢物直接或间接的影响了林下人参的品质。本研究不仅揭示了林下栽培人参和农田栽培人参根际土壤代谢物的差异,而且为提高林下栽培人参品质、探索克服林下人参栽培连作障碍的新途径以及提升林下人参药用价值和食用价值提供了科学参考。并为中国东北地区林下人参产业发展及乡村振兴战略的实施提供了新思路和方法。
Differences in Inter-root Soil Metabolites of Ginseng Under Different Cultivation Patterns
The study aims to investigate the differences in inter-root soil metabolites between forest-grown ginseng(PSM)and ginseng grown on farmland(NPSM),and provide a scientific reference for improving the quality of forest-grown ginseng.The study employed GC-MS/MS assay and molecular biology analysis to compare the inter-root soil metabolites of the two types of ginseng.We detected a total of 68 metabolites in the inter-root soil of wild-like forest cultivated ginseng and farmland cultivated ginseng.Among these metabolites,17 were significantly different,with 14 up-regulated and 3 down-regulated metabolites.The detected metabolites included sugars,lipids,organic acids,esters and other substances.Sugars accounted for 47% of the total significant differential metabolites,which was the largest category of differential metabolites.These different metabolites in rhizosphere soil could directly or indirectly affect the quality of forest ginseng.This study examined the differences in inter-root soil metabolites between forest-cultivated ginseng and farmland-cultivated ginseng.The findings provided a scientific reference for improving the quality of forest-cultivated ginseng and exploring the cultivation environment of forest ginseng to overcome the obstacles of ginseng continuous cropping,improving the medicinal and edible value of forest ginseng and to provide new ideas and methods for the rural revitalization strategy of the understory ginseng industry in the northeast region of China.

understory ginsenginter-root soilcontinuous crop barriersmetabolic differentialsfarmland cultivation of ginsengGc-Ms/Msquality improvement

焦金龙、施蕊、汪永贵、王慧玲、张澳、严星茹、王澍、何霞红

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云南省林下资源保护与利用重点实验室,西南林业大学园林与园艺学院,昆明 650224

云南农业大学植物保护学院,昆明 650500

林下栽培人参 根际土壤 连作障碍 代谢差异物 农田栽培人参 GC-MS/MS 品质提升

国家自然科学基金国家重点研发计划项目云南省重大科技专项昆明市科技揭榜制项目

322607202021YFD1000202202102AE0900422021JH002

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子:0.891
ISSN:1000-6850
年,卷(期):2024.40(20)