Distribution of Six Rice Blast Resistance Genes in Main Rice Varieties in Menghai County of Yunnan Province
The purpose of this study is to clarify the composition of the cloned blast resistance genes Pi2,Pi9,Piz-t,Pi50/Pigm,Pita-2,and Pib among the major cultivated rice cultivars in Menghai County.Eighteen major cultivated rice cultivars grown in Menghai County were selected as experimental materials,and molecular detection was carried out using gene-specific molecular markers for the aforementioned 6 resistance genes.The results showed that the distribution frequency of the resistance gene Pib was the highest,with a detection rate of 77.78% among these tested cultivars.The detection rates of Pi2,Pita-2,and Piz-t were 22.22% ,22.22% ,and 11.11% ,respectively.Resistance genes Pi9 and Pi50/Pigm were not detected in the tested cultivars.Among the tested cultivars,there were 8 varieties(each accounting for 44.44% )with 2 resistance genes and 1 resistance gene detected.There was no any one of 6 known resistance genes was detected in 2 cultivars,which was accounting for 11.11% of all tested cultivars.The results indicate that the genetic background of resistance in major rice cultivars cultivated in Menghai County is relatively narrow.It is necessary to introduce new and effective resources of resistance to broaden the resistance spectrum of rice cultivars to rice blast.
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