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猕猴桃黑头病菌的生物学特性及其致病性研究

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明确猕猴桃黑头病病原菌的生物学及致病特性,研究不同培养条件下菌体的生长特性,及其对不同品种猕猴桃的致病性差异,为深入开展侵染机理、流行规律及病害防治关键技术等研究提供理论依据.结果表明,猕猴桃黑头病的病原菌Diaporthe eres在15~30℃、pH 5~11条件下均能生长,最适温度28℃,对高温敏感,高于35℃时菌体生长速度显著降低.菌体在多种碳源中均能生长,最适碳源为葡萄糖.更易利用有机氮源,最适氮源为酵母浸粉.钙离子对病原菌生长具有促进作用,菌体生长速度与钙离子含量呈正比.菌体不能产生有性孢子,只能以无性分生孢子进行繁殖.对'翠香'、'徐香'、'瑞玉'猕猴桃具有明显的致病性,但是不侵染'海沃德'、'亚特'、'秦美'猕猴桃.
Biological Characteristics and Pathogenicity of Kiwifruit Black Spot Pathogen
In order to confirm the biological and pathogenic characteristics of the pathogenic fungi of black spot on kiwifruit,the biological characteristics of the strain under different culture conditions and their pathogenicity differences to varieties of kiwifruits were studied.It will provide theoretical basis for further research on infection mechanism,epidemic rules and key technologies of disease prevention and control.The results showed that Diaporthe eres,the pathogen of kiwifruit-black spot,could grow at 15-30℃ and pH 5-11.The optimum temperature was 28℃,and it was sensitive to high temperature.The growth rate of the fungus decreased significantly when the temperature was higher than 35℃.The strain could grow in different carbon sources,the most suitable carbon source was glucose.It was easier to use organic nitrogen source,and the most suitable nitrogen source was yeast extract powder.Calcium could promote the growth of the strain,and the growth rate was proportional to the content of calcium.The strain could only reproduce conidia,but could not produce ascospores.The strain had obvious pathogenicity to'Cuixiang','Xuxiang'and'Ruiyu'kiwifruits,but could not infect'Hayward','Yate'and'Qinmei'kiwifruits.

black spot on kiwifruitDiaporthe eresbiological characteristicspathogenicityculture conditionscalcium ion effects

付博、王家哲、任平、李浩、常青、李英梅、张锋

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陕西省生物农业研究所,西安 710043

周至县植保植检站,西安 710400

猕猴桃黑头病 间座壳属 生物学特性 致病性 培养条件 钙离子影响

西安市科技局科技项目陕西省重点研发计划

21NYYF00262021NY-060

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子:0.891
ISSN:1000-6850
年,卷(期):2024.40(22)