Explanation of Integrative Analysis of Metabolome and Transcriptome for Anthocyanins Synthesis Mechanism in Cassava Leaves
Cassava is the sixth largest staple food crop in the world,which storage root is rich in starch while lack of proteins,anthocyanins and carotenes and so on.Two types of leaves of cassava with different color(FL and PL)were selected as materials to explore the mechanism of anthocyanin biosynthesis by combining with transcriptome and metabolome in this work.The transcriptome results showed that there were 6864 differentially expressed genes(DEGs)including 4112 up-regulating and 2752 down-regulating in PL compare with FL.The metabolome results showed that there were 26 significantly different metabolites(SDMs)in PL compare with FL,and 21 of them belonged to anthocyanins.The result of combination of transcriptome and metabolome showed that there were seven DEGs related to anthocyanin biosynthesis and all of them were positively correlated to anthocyanin biosynthesis.The expression of MeANS1 showed the most difference.It provided candidate genes for elucidating the mechanism of anthocyanin biosynthesis in cassava and laid a scientific foundation for enhancement content of anthocyanin in future.