复合抗生素对生菜种子萌发及土壤酶活性的影响
Impact of Compound Antibiotics on Lettuce Seed Germination and Soil Enzyme Activity
郭佳祎 1于一雷 2李文彦 3吕翠翠 3刘超杰1
作者信息
- 1. 北京农学院植物科学技术学院,北京 102206
- 2. 中国科学院雄安创新研究院(筹),河北雄安 071899;地下水污染控制与修复教育部工程研究中心/北京师范大学,北京 100875
- 3. 中国科学院雄安创新研究院(筹),河北雄安 071899
- 折叠
摘要
为探讨抗生素和灌溉水源变化对生菜、土壤的影响,以及其在培养第3、7 d时间上的变化,设计添加11种常见抗生素的复合污染水平(0、2.2、5.5、11 mg/kg)和2种不同灌溉水源的种子萌发及胁迫试验,并在发芽期间收集2次样品.结果表明,大部分生菜种子萌发指标(如种子的发芽势、发芽率、鲜重和苗长)在处理组与对照组间没有显著差异.在添加11 mg/kg浓度抗生素且灌溉水源为再生水时生菜种子的根长被显著抑制.采样期内(培养的第3、7d),抗生素的添加和灌溉水源的变化没有扰乱生菜种子的抗氧化系统正常工作.培养第3~7 d,大多数处理组的土壤脲酶、土壤碱性磷酸酶活性有所上升,部分处理组与对照组之间存在显著差异,土壤酶活性相比植物萌发指标和植物酶活性对抗生素的添加更为敏感.
Abstract
To investigate the effects of antibiotics and changes in irrigation water source on lettuce,soil and their changes in time on the 3rd and 7th day of incubation,seed germination and stress tests were designed with the addition of 11 common antibiotics at compound contamination levels(0,2.2,5.5 and 11 mg/kg)and two different irrigation water sources,and samples were collected twice during germination.The results showed that most of the lettuce seed germination indices(e.g.,seed germination potential,germination percentage,fresh weight,and seedling length)were not significantly different in the treatment group compared to the control group.Root length of lettuce seeds was significantly inhibited when the concentration of addition of antibiotics was 11 mg/kg and the source of irrigation water was reclaimed water.During the sampling period(3rd and 7th day of incubation),the addition of antibiotics and the change in irrigation water source did not disturb the normal functioning of the antioxidant system of lettuce seeds.Soil urease and soil alkaline phosphatase activities increased in most of the treatment groups from the 3rd to the 7th day of incubation,and there was a significant difference between some treatment groups and the control group.Soil enzyme activities were more sensitive to the addition of antibiotics than plant germination indexes and plant enzyme activities.
关键词
抗生素/复合污染/生菜/种子萌发/土壤酶活性/再生水/生态风险Key words
antibiotics/composite pollution/lettuce/seed germination/antioxidant enzyme/reclaimed water/ecological risk引用本文复制引用
基金项目
地下水污染控制与修复教育部工程研究中心开放基金项目(GW203312)
河北省重大科技成果转化专项(23267201Z)
出版年
2024