Impact of Compound Antibiotics on Lettuce Seed Germination and Soil Enzyme Activity
To investigate the effects of antibiotics and changes in irrigation water source on lettuce,soil and their changes in time on the 3rd and 7th day of incubation,seed germination and stress tests were designed with the addition of 11 common antibiotics at compound contamination levels(0,2.2,5.5 and 11 mg/kg)and two different irrigation water sources,and samples were collected twice during germination.The results showed that most of the lettuce seed germination indices(e.g.,seed germination potential,germination percentage,fresh weight,and seedling length)were not significantly different in the treatment group compared to the control group.Root length of lettuce seeds was significantly inhibited when the concentration of addition of antibiotics was 11 mg/kg and the source of irrigation water was reclaimed water.During the sampling period(3rd and 7th day of incubation),the addition of antibiotics and the change in irrigation water source did not disturb the normal functioning of the antioxidant system of lettuce seeds.Soil urease and soil alkaline phosphatase activities increased in most of the treatment groups from the 3rd to the 7th day of incubation,and there was a significant difference between some treatment groups and the control group.Soil enzyme activities were more sensitive to the addition of antibiotics than plant germination indexes and plant enzyme activities.