Identification of Pestalotiopsis microspora Causing Fruit Spot on Guava and Laboratory Screening of Fungicides
In order to clarify the pathogen causing guava fruit spot disease and to control the disease,the pathogens from guava leaves and fruit with typical symptoms were collected for isolation and purification.Based on tissue isolation method,phylogenetic analysis,morphological characters and Koch's postulates,the pathogen was identified as Pestalotiopsis microspora.Inhibitory activities of six fungicides to P.microspora Guava A,Guava B and Guava C were determined using mycelial growth method.The result indicated 450 g/L prochloraz EW had the best inhibitory effect on the mycelial growth,with an EC50 values of 0.0019 μg/mL;the following was 60％pyraclostrobin·fluxapyroxad SC,75％trifloxystrobin·tebuconazole WG and 325 g/L difenoconazole·azoxystrobin SC,with EC50 values of 0.1479 pig/mL,0.1697 μg/mL and 0.1739 μg/mL.The result provides a reference for the control of fruit spot disease on guava in field.
guavaleaf spotfruit spotPestalotiopsis microsporafungicideinhibitory activity in vitro
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