摘要
研究旨在阐明云南省马关县红果参品系果实花色苷的紫外-可见光谱和含量,为马关县乃至云南省的红果参品种选育以及果实的开发、利用和营销提供依据.以马关县红果参品系'文黑果1号'～'文黑果8号'为材料,基于在200～700 nm范围内的连续扫描检测了果实花色苷的紫外-可见光谱,用分光光度法测定了果皮、果肉和果实的总花色苷含量(TAC).结果表明,8个红果参品系果实花色苷1％浓盐酸-甲醇提取液的紫外-可见光谱中,在可见光区和紫外光区分别存在1个特征性吸收带,在可见光区的最大吸收峰波长为537.0 nm.果皮、果实TAC均最高的是'文黑果2号',分别约为(3.546±0.424)、(1.758±0.070)A537.o/(g·FW);TAC果皮/TAC果实最高的是'文黑果 5 号',约为 2.237±0.096;TAC 果皮/TAC果肉最高的是'文黑果6号',约为14.911±1.729;'文黑果7号'的果肉TAC、TAC 果肉/TAC果实均最高,分别约为0.809±0.010A5370/(g FW)、1.284±0.056.此外,所有TAC相关指标在8个品系间的差异均达到极显著水平.
Abstract
This research aimed to clarify the ultraviolet-visible(UV-vis)spectra and contents of the fruit anthocyanins of the Cyclocodon lancifolius strains in Maguan County,Yunnan Province,providing a basis for the breeding,development,utilization and marketing of the fruits of the C.lancifolius in the county,and even in Yunnan Province.The C.lancifolius strains,i.e.,'Wenheiguo No.1'-'Wenheiguo No.8',in the county were employed as the test materials.The UV-vis spectra of the fruit anthocyanins were determined based on the continuous scanning in the range of 200-700 nm,and the total anthocyanin contents(TACs)of the fruits including the peels and flesh were evaluated by using spectrophotometry.The results showed that,in the UV-vis spectra of 1％concentrated hydrochloric acid-methanol extracts of the fruit anthocyanins of the eight C.lancifolius strains,one specific absorption band emerged in the visible and ultraviolet light regions,respectively,and the maximum absorption peak wavelength in the visible light region was 537.0 nm.'Wenheiguo No.2'had the highest TACs for both the peels and the whole fruits,i.e.,about(3.546±0.424)A537.0/(g·FW)and(1.758±0.070)A537.0/(g·FW),respectively,'Wenheiguo No.5'held the highest TAC ratio of the peels to the whole fruits,i.e.,about 2.237±0.096,'Wenheiguo No.6'possessed the highest TAC ratio of the peels to the flesh,i.e.,about 14.911±1.729,and the flesh TAC and the TAC ratio of the flesh to the whole fruits of'Wenheiguo No.7'were both the highest,i.e.,about(0.809±0.010)A537.0/(g·FW)and 1.284±0.056,respectively.In addition,the differences of all TAC-related indexes among the eight strains reached extremely significant.
基金项目
文山壮族苗族自治州农业科学院资助项目(WYHZ2023-01)
文山壮族苗族自治州财政局资助项目(46)
国家科技期刊平台
NSTL
万方数据