一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位
Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice
刘晓敏 1孙志广 1迟铭 1邢运高 1徐波 1李景芳 1刘艳 1卢百关 1王宝祥 1徐大勇1
作者信息
- 1. 连云港市农业科学院/江苏省现代作物生产协同创新中心,江苏连云港 222000
- 折叠
摘要
本研究旨在对水稻黄绿叶突变体ygl-9108进行表型分析和基因定位,为后续图位克隆该叶色相关基因奠定基础.以黄绿叶突变体ygl-9108为试材,利用突变体ygl-9108和'五山丝苗'杂交的F2群体进行基因定位.结果表明,与野生型相比,突变体的株高、有效穗数、穗长、每穗粒数、结实率、粒宽、千粒重和单株谷重均显著下降,下降比例分别为21.5％、21.2％、14.6％、19.2％、11.0％、10.2％、12.9％和52.2％.透射电镜观察显示,突变体叶肉细胞的叶绿体数量大量减少,类囊体结构异常.遗传分析表明,突变体的表型由一个隐性核基因控制.利用图位克隆方法,ygl-9108被定位于水稻第11号染色体两InDel标记11Y39和11Y45之间,物理距离约为147 kb,该区间内未见有叶色相关基因的报道,表明ygl-9/08是一个新的黄绿叶调控基因.本研究结果为ygl-9108的克隆和功能分析奠定了坚实的基础.
Abstract
The research aims to lay the foundation for map-based cloning of the leaf color related gene by analyzing the phenotypic characteristics of a rice yellow-green leaf mutant ygl-9108 and mapping the mutant locus.A yellow-green leaf mutant,named as ygl-9108,was selected as experimental material,and the gene was mapped by using the F2 population derived from the cross between the ygl-9108 mutant and'Wushansimiao'.The results showed that the plant height,effective panicle number,panicle length,grain number per panicle,seed setting rate,grain width,1000-grain weight,and grain weight per plant of the ygl-9108 mutant were all significantly reduced by 21.5％,21.2％,14.6％,19.2％,11.0％,10.2％,12.9％,and 52.2％,respectively,compared with those of the wild type.The observation by transmission electronic microscope(TEM)demonstrated that the number of chloroplasts in mesophyll cells of ygl-9108 plants was greatly reduced,and the lamellar structure of chloroplasts was abnormal.Genetic analysis revealed that the phenotype of mutant was controlled by a single recessive nuclear gene.Using a map-based cloning strategy,ygl-9108 was mapped to a 147 kb region on chromosome 11 between two InDel markers,11Y39 and 11Y45.No gene related to leaf color has been reported previously in this interval,indicating that ygl-9108 is a new leaf color-related gene.This study lays a solid foundation for further research on gene cloning and function analysis of ygl-9108.
关键词
水稻/黄绿叶突变体/ygl-9108/表型鉴定/农艺性状/基因定位/透射电镜/叶绿体/隐性核基因/图位克隆Key words
Oryza sativa L./yellow-green leaf mutants/ygl-9108/phenotypic identification/agronomic trait/gene mapping/transmission electron microscope/chloroplast/recessive nuclear gene/map-based cloning引用本文复制引用
基金项目
国家现代农业产业技术体系建设专项(CARS-01-01A)
江苏省种业振兴揭榜挂帅项目(JBGS[2021]039)
连云港市财政专项(QNJJ2311)
&&(QNJJ2203)
出版年
2024
NETL
NSTL
万方数据