一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位

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中文摘要：本研究旨在对水稻黄绿叶突变体ygl-9108进行表型分析和基因定位,为后续图位克隆该叶色相关基因奠定基础.以黄绿叶突变体ygl-9108为试材,利用突变体ygl-9108和'五山丝苗'杂交的F2群体进行基因定位.结果表明,与野生型相比,突变体的株高、有效穗数、穗长、每穗粒数、结实率、粒宽、千粒重和单株谷重均显著下降,下降比例分别为21.5％、21.2％、14.6％、19.2％、11.0％、10.2％、12.9％和52.2％.透射电镜观察显示,突变体叶肉细胞的叶绿体数量大量减少,类囊体结构异常.遗传分析表明,突变体的表型由一个隐性核基因控制.利用图位克隆方法,ygl-9108被定位于水稻第11号染色体两InDel标记11Y39和11Y45之间,物理距离约为147 kb,该区间内未见有叶色相关基因的报道,表明ygl-9/08是一个新的黄绿叶调控基因.本研究结果为ygl-9108的克隆和功能分析奠定了坚实的基础.

外文标题：Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice

外文摘要：The research aims to lay the foundation for map-based cloning of the leaf color related gene by analyzing the phenotypic characteristics of a rice yellow-green leaf mutant ygl-9108 and mapping the mutant locus.A yellow-green leaf mutant,named as ygl-9108,was selected as experimental material,and the gene was mapped by using the F2 population derived from the cross between the ygl-9108 mutant and'Wushansimiao'.The results showed that the plant height,effective panicle number,panicle length,grain number per panicle,seed setting rate,grain width,1000-grain weight,and grain weight per plant of the ygl-9108 mutant were all significantly reduced by 21.5％,21.2％,14.6％,19.2％,11.0％,10.2％,12.9％,and 52.2％,respectively,compared with those of the wild type.The observation by transmission electronic microscope(TEM)demonstrated that the number of chloroplasts in mesophyll cells of ygl-9108 plants was greatly reduced,and the lamellar structure of chloroplasts was abnormal.Genetic analysis revealed that the phenotype of mutant was controlled by a single recessive nuclear gene.Using a map-based cloning strategy,ygl-9108 was mapped to a 147 kb region on chromosome 11 between two InDel markers,11Y39 and 11Y45.No gene related to leaf color has been reported previously in this interval,indicating that ygl-9108 is a new leaf color-related gene.This study lays a solid foundation for further research on gene cloning and function analysis of ygl-9108.

外文关键词：

Oryza sativa L.yellow-green leaf mutantsygl-9108phenotypic identificationagronomic traitgene mappingtransmission electron microscopechloroplastrecessive nuclear genemap-based cloning

作者：

刘晓敏、孙志广、迟铭、邢运高、徐波、李景芳、刘艳、卢百关、王宝祥、徐大勇

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作者单位：

连云港市农业科学院/江苏省现代作物生产协同创新中心,江苏连云港 222000

关键词：

水稻黄绿叶突变体 ygl-9108 表型鉴定农艺性状基因定位透射电镜叶绿体隐性核基因图位克隆

基金：

国家现代农业产业技术体系建设专项江苏省种业振兴揭榜挂帅项目连云港市财政专项&&

项目编号：

CARS-01-01AJBGS[2021]039QNJJ2311QNJJ2203

出版年：

2024

中国农学通报

中国农学会

中国农学通报

CSTPCD

影响因子：0.891

ISSN：1000-6850

年,卷(期)：2024.40(26)