Regeneration System of Codonopsis pilosula Resources:Seed Sterilization Methods
As an authentic medicinal material in Shanxi Province,Codonopsis pilosula(Franch.)Nannf.is well-known throughout the country for its quality and curative effect.In order to effectively protect the high-quality germplasm resources of Codonopsis pilosula,an efficient regeneration system of Codonopsis pilosula was constructed.In this experiment,Codonopsis pilosula seeds were used as test materials,using 4 different treatment methods,after isolation and wrapping,two different sterilizers were used to treat mercury and sodium hypochlorite,respectively,and the damage to the seeds by different treatments was observed to determine the best sterilization method.The results showed that under different sterilization treatments,the seed contamination rate was 0-92.7％ ,and the survival rate was 0.8％ -91.3％ .Based on the combination of pollution rate and survival rate,treatment 29 was selected as the best result and followed by treatments 30.After wrapping the seeds in method 4,the best effect was achieved after 60-120 s of mercury sterilization,and the survival rate of the seeds was 82.00％ -91.3％ ,while the contamination rate was 2.17％ -3.83％ .This experiment establishes a seed sterilization method suitable for the regeneration system of Codonopsis pilosula and lays the foundation for biotechnological breeding of Codonopsis pilosula.
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