首页|异源表达甜菜BvHSP18.2基因增强拟南芥对镉胁迫的耐受性研究

异源表达甜菜BvHSP18.2基因增强拟南芥对镉胁迫的耐受性研究

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为进一步分析和验证甜菜BvHSP18.2基因(LOC104903994)受镉胁迫调控的功能,本研究用RT-PCR技术克隆了甜菜BvHSP18.2基因,并通过植物表达载体构建以及拟南芥的遗传转化,测得异源表达目的基因的拟南芥植株在不同浓度镉胁迫下的表型及生理变化数据.研究结果表明,随着镉胁迫浓度的增加,异源表达BvHSP18.2基因的拟南芥无论在根长还是鲜重方面均具有优势:50μmol/L镉胁迫下平均根长相差最大,为1.1 cm,600 μmol/L镉胁迫下平均鲜重相差最大,为0.015 g.同时,BvHSP18.2基因的过表达可有效提高拟南芥体内SOD和POD活性,300 μmol/L镉胁迫下SOD活性最高,为657.7266953 U/g;600 μmol/L镉胁迫下POD活性最高,为野生型拟南芥的1.91倍.BvHSP18.2基因的过表达增强了拟南芥在镉胁迫下的耐受性,推测该基因在植物对镉胁迫的适应机制中具有重要的作用.
Heterologous Expression of BvHSP18.2 Gene in Sugar Beet Enhancing Tolerance to Cadmium of Arabidopsis thaliana
In order to further analyze and verify the function of the sugar beet BvHSP18.2 gene(LOC104903994)regulated by cadmium stress,we cloned the sugar beet BvHSP18.2 gene by RT-PCR in this study,and measured the phenotypic and physiological phenotypes and physiological changes of Arabidopsis thaliana plants heterologously expressing the target gene under different concentrations of cadmium stress by plant expression vector construction as well as Arabidopsis thaliana genetic transformation.The phenotypic and physiological changes of Arabidopsis thaliana plants heterologously expressing the target gene under different concentrations of Cd were measured.The results showed that with the increase of cadmium stress concentration,Arabidopsis thaliana plants heterologously expressing the BvHSP18.2 gene had an advantage in both root length and fresh weight.The largest difference was 1.1 cm in average root length under 50 pmol/L cadmium stress,and the largest difference was 0.015 g in average fresh weight under 600 pmol/L cadmium stress,and the overexpression of the BvHSP18.2 gene could effectively increase the S-phase of Arabidopsis thaliana plants under different concentrations of cadmium stress.The overexpression of BvHSP18.2 gene enhanced the tolerance of Arabidopsis thaliana under cadmium stress,and it was hypothesized that this gene played an important role in the adaptation of plants to cadmium stress.

sugar beetArabidopsis thalianaBvHSP18.2 geneheterologous expressioncadmium stressfunctional analysisgene cloningsod activitypod activitytolerancegenetic transformation

仲维婷、张琼、兴旺、刘大丽

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国家甜菜种质中期库/黑龙江大学,哈尔滨 150080

黑龙江省普通高等学校甜菜遗传育种重点实验室/黑龙江大学现代农业与生态环境学院,哈尔滨 150080

甜菜 拟南芥 BvHSP18.2基因 异源表达 镉胁迫 功能分析 基因克隆 SOD活性 POD活性 耐受性 遗传转化

财政部和农业农村部"国家现代农业产业技术体系(糖料)建设"项目甜菜种质资源安全保存项目内蒙古自治区"揭榜挂帅"项目黑龙江省高校科研业务费项目黑龙江省自然科学基金

CARS-170102192308412022JBGS00292022-KYYWF-1070LH2023C090

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2024.40(29)