首页|工业大麻内生真菌HDM07挥发性成分分析

工业大麻内生真菌HDM07挥发性成分分析

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旨在开发利用工业大麻内生真菌HDM07挥发性成分，探索工业大麻内生真菌HDM07次生代谢产物生物合成途径。以工业大麻内生真菌HDM07为研究对象，采用GC-MS法和检索质谱数据库分析鉴定内生真菌HDM07发酵液和菌丝体中的挥发性成分，采用形态学观察法和18SrDNA序列分析法鉴定菌种。结果表明，经GC-MS法和质谱数据库检索分析，从内生真菌HDM07发酵液中鉴定出11种挥发性成分，在内生真菌HDM07菌丝体中鉴定出14种挥发性成分，工业大麻叶中鉴定了 12种成分，空白对照品溶液(PDB培养基)中鉴定了 7种成分;经鉴定内生真菌HDM07为链格孢菌(Alternaria alternate)。上述结果表明，HDM07发酵液挥发性成分与菌丝体中挥发性成分两者的合成途径既有联系又有区别。内生真菌HDM07的发酵液及菌丝体中均含有与工业大麻相同或相似的挥发性成分，也含有其特有的挥发性成分，说明该菌株与宿主植物工业大麻在合成挥发性成分方面具有一定的相关性。
Analysis of Volatile Components of Endophytic Fungus HDM07 in Cannabis sativa L.
This study aims to exploit volatile components of endophytic fungus HDM07 isolated from Cannabis sativa L.,and to explore the biosynthetic pathway of secondary metabolites from HDM07.The research took the endophytic fungus HDM07 obtained from Cannabis sativa L.as the subject.GC-MS and retrieval mass spectrometry database were employed to identify the volatile components in both the fermentation broth and mycelium of HDM07.Morphological observation and 18S rDNA sequence analysis were conducted for strain identification.Results indicated that the method of GC-MS and mass spectrometry analysis,along with database retrieval from endophytic fungi HDM07,determined 11 volatile components in the fermented liquid.Furthermore,fungus HDM07 revealed 14 volatile constituents in mycelium and Cannabis sativa L.leaf identified 12 elements.In a blank reference substance solution(PDB medium),appraisal revealed 7 ingredients.The endophytic fungus HDM07 was identified as Alternaria alternate.These findings suggest that the synthesis pathways of volatile components in HDM07 fermentation broth and mycelia are both related yet distinct.Both the fermentation broth and mycelium of endophytic fungus HDM07 contained similar or identical volatile components to those found in Cannabis sativa L.,and also contained unique ones,indicating a certain correlation between the strain and its host plant's synthesis of volatile components.

Cannabis sativa L.endophytic fungus HDM07 from Cannabis sativa L.18S rDNA sequence analysisGC-MSvolatile component

邢翔宇、丛添玥、关昕、祁可香、李宛儒、张赫、尤梦瑶、吴桐、郑春英

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黑龙江大学,生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,哈尔滨 150080

黑龙江大学,生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080

工业大麻 工业大麻内生真菌HDM07 18S rDNA序列分析 气相色谱质谱法 挥发性成分

黑龙江省种业创新发展资金项目黑龙江省现代农业产业技术协同创新体系项目黑龙江大学横向项目黑龙江大学横向项目黑龙江大学横向项目

20230202YYM19STX-1211922311423113

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2024.40(30)