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丹参根腐病病原菌及其生防菌的筛选

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本研究旨在筛选用于丹参根腐病防治的生物防治菌株。实验以山东省日照市、临沂市、泰安市发病的丹参及健康丹参的根际土为试材。采用组织分离法从发病丹参中分离病原菌，采用稀释涂布法和平板对峙法从根际土中筛选生防菌，利用形态学和16S rRNA进行鉴定，并在离体丹参上进行防效验证。结果表明，从发病丹参上分离出的病原菌RBT-2-1为木贼镰刀菌(Fusarium equiseti)，从根际土中筛选出的生防菌i为绿针假单胞菌橙色亚种(Pseudomonas chlororaphis subsp。aurantiaca)。i对TBG-2-1的抑菌率为66。67％，i在离体丹参上对TBG-2-1的防治效果预防组均高于治疗组，且预防组中追加i菌液的防效最好，为44。45％，比预防组中未追加i菌液的处理提高了 1。6倍，比治疗组提高了 7。0倍。由此可知，i的接种顺序及使用次数会影响对TBG-2-1的防效。本研究为后期大田试验提供参考依据。
Root Rot Pathogens of Salvia miltiorrhiza and Screening of Biocontrol Bacteria
To screen the biocontrol bacteria of Salvia miltiorrhiza root rot,the infected S.miltiorrhiza and rhizosphere soil from healthy S.miltiorrhiza in Rizhao,Linyi,and Tai'an cities of Shandong Province were used as test materials.The tissue separation method was used to isolate the root rot pathogen from infected S.miltiorrhiza.The gradient dilution plate method and disk diffusion method were used to screen biocontrol bacteria from rhizosphere soil.Identification was achieved through the use of morphological characteristics and 16S rRNA,and control effect testing was carried out on the detached S.miltiorrhiza.The results showed that the pathogen RBT-2-1 isolated from infected S.miltiorrhiza was Fusarium equiseti,and the biocontrol bacterium i screened from rhizosphere soil was Pseudomonas chloriraphis subsp.aurantiaca.The antibacterial rate of i against TBG-2-1 was 66.67％.The control effect of i against TBG-2-1 on the detached S.miltiorrhiza in the prevention group was higher than in the treatment group.Furthermore,the addition of i in the prevention group resulted in the best control effect,reaching 44.45％.The prevention group of adding i was 1.6 times higher than the prevention group without addition,and 7.0 times higher than the treatment group.It can be concluded that the sequence and frequency of inoculation with i will affect the prevention efficacy of TBG-2-1.This study serves as a guide for future field experiments.

root rot of Salvia miltiorrhizaFusarium equisetiPseudomonas chlororaphis subsp.aurantiacabiocontrol bacteriumdisk diffusion methodprevention and control effectvaccination sequenceusage frequency

袁梦蕾、崔明悦、张子通、刘亚钰、刘方春、马海林、刘幸红、燕丽萍

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山东沃恩生物科技有限公司,济南 250014

山东省林业科学研究院,济南 250014

丹参根腐病 木贼镰刀菌 绿针假单胞菌橙色亚种 生防菌 平板对峙法 防治效果 接种顺序 使用次数

济南市科技型中小企业创新能力提升工程泰山学者青年专家奖励计划

2024

中国农学通报
中国农学会

中国农学通报

CSTPCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2024.40(30)