Improvement of Salt Tolerance in Cotton Seed Germination by Ascorbic Acid and Its Physiological Mechanism
The study aims to explore physiological mechanisms of salt tolerance in cotton during seed germination stage.Using'Xinluzao NO.61',a major local cotton variety,as the test material,the effects of ascorbic acid(AsA)soaking on key germination characteristics and physiological indicators of cotton seeds were analyzed under different types of salt stress.The results showed that the germination of cotton seeds decreased with increasing salt stress concentration;Under NaCl,Na2SO4,and Na2CO3+NaHCO3 stress,the soaking with AsA enhanced the germination characteristics of cotton seeds,among them,the germination rate of cotton seeds increased the most,increasing by 55.32％,56.10％,and 44.0％compared to the control,respectively,and the optimal AsA soaking concentration for improving salt tolerance was selected as 0.10 mmol/L,0.10 mmol/L,and 0.15 mmol/L;during the germination process under salt stress,the activities of SOD,POD and CAT,and the contents of soluble protein and soluble sugar in cotton seeds soaked with AsA increased,while the content of MDA and H2O2 decreased,and all showed significant differences from the control group.It is concluded that AsA soaking can improve the salt tolerance of cotton seed during germination,as it enhances the main antioxidant enzyme activity,increases the content of osmotic substances,and alleviates membrane lipid peroxidation of cotton seed.
万方数据
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