Influence of Altitude on Quality and Microbial Community Structure of Yunnan Produced Cigar Tobacco Leaves
To investigate the impact of planting altitude on the quality and microbial community structure of Yunnan produced cigar,the sensory quality,neutral aroma components,and microbial community structure of the main cigar variety'Yunxue No.39'cultivated at four different altitudes in Yuxi City were examined.The results showed that the sensory quality scores of'Yunxue No.39'decreased in the order of Ganzhuang(medium-low altitude),Lijiang(low altitude),Wadie(medium-high altitude),and Yinyuan(high altitude).The samples from medium-low altitude areas had sufficient aroma quantity,rich aroma types,less offensive odor,weak irritation,and good aftertaste.A total of 31 major neutral aroma components were detected,and the content of these components from high to low was carotenoid degradation products,chlorophyll degradation products,phenylalanine conversion products,and cembrane degradation products,with a large variation in the content of each monomer component.There were differences in the structural composition and relative abundance of the surface bacterial communities of cigar tobacco leaves at different altitudes.The Ganzhuang samples at medium-low altitude had the largest total number of OTUs,the largest number of unique OTUs,and the richest bacterial community diversity.The unidentified-chloroplast and Chryseobacterium were the key genera contributing to the differences in the bacterial community structure of tobacco leaves.The metabolic pathways of the samples at different planting altitudes were mainly signal transduction and cell processes,amino acid metabolism,fatty acid metabolism,genetic information processing,and carbohydrate metabolism,but the abundances of these metabolic pathways differed at different altitudes.This study indicated that different altitudes had significant impact on the quality of cigar tobacco leaves and the structure of microbial colonies.
altitudeYunnan produced cigarsensory qualityneutral aromatic componentsmicrobial community structuremetabolic pathway
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