陕西关中枣区枣疯病介体昆虫种类鉴定及种群消长动态分析
Species Identification and Population Dynamics of Potential Vectors of Jujube Witches'Broom in Guanzhong,Shaanxi Province
翟颖妍 1张锋 1田天祺 1杨艺炜 1曹东平 2洪波1
作者信息
- 1. 陕西省生物农业研究所,西安 710043
- 2. 榆林市林业工作站,陕西 榆林 719000
- 折叠
摘要
为预防和控制陕西关中枣区枣疯病,明确枣疯病潜在的介体昆虫种类,确定主要叶蝉的种群消长动态,于陕西省大荔县3块枣疯病发病严重的枣园,利用黄板诱捕法,从2022年4-10月对枣园的潜在介体昆虫进行调查和鉴定,并基于16SrDNA基因对昆虫枣疯病植原体携带率进行分析.发现潜在介体昆虫11种,包括多彩斑翅叶蝉、片突菱纹叶蝉、凹缘菱纹叶蝉、一点木叶蝉、小绿叶蝉、条沙叶蝉、茶扁叶蝉、异色对纹叶蝉、褐三刺角蝉、灰飞虱和绿盲蝽.不同昆虫的种群消长动态表明,多彩斑翅叶蝉、片突菱纹叶蝉和凹缘菱纹叶蝉为枣园的优势种,相对丰度分别为58.38%、15.68%和10.32%.叶蝉成虫的数量呈先上升后下降的趋势,多彩斑翅叶蝉有4个数量高峰,片突菱纹叶蝉和凹缘菱纹叶蝉均有2个数量高峰.枣疯病植原体PCR检测结果表明,片突菱纹叶蝉、凹缘菱纹叶蝉和灰飞虱样本中枣疯病植原体携带率分别为13.33%、12.67%和10.00%,其他昆虫中未检测出枣疯病植原体.本研究首次在灰飞虱体内检测到枣疯病植原体,表明灰飞虱可能为潜在的枣疯病介体昆虫.对介体昆虫的监测与防治有利于控制枣疯病的流行与蔓延,研究旨在为以阻断枣疯病传播链为目的的介体昆虫防治提供参考.
Abstract
In order to preventing and controlling the jujube witches'broom(JWB),the species identification and population dynamics of potential insect vectors transmitting JWB phytoplasma were performed and analyzed in jujube production area of Guanzhong area,Shaanxi province.The yellow sticky traps were utilized to survey and identify the insect vectors in the jujube orchards in Dali County,from April to October 2022.The rate of JWB phytoplasma carriage by potential vectors was analyzed based on 16SrDNA gene.Eleven species of potential vectors were identified,including Hishimonus lamellatus Cai et Kuoh,Hishimonus sellatus Uhler,Psammotettix striatus Linnaeus,Phlogotettix Cyclops Mulsant et Rey,Chanohirata theae Matsumura,Tautoneura polymitusa Oh&Jung,Zygina discolor Horváth,Empoascas pp.,Tricentrus brunneus Funkhouser,Laodelphax striatellus Fallén and Apolygus lucorum Meyer-Dür.Population dynamics of different insects showed that T.polymitusa,H.lamellatus and H.sellatus were the dominant species in jujube orchards,with relative abundances of 58.38%,15.68%and 10.32%,respectively.The number of leafhopper adults showed a trend of increasing and then decreasing.There were four population peaks for T.polymitusa,and two peaks for H.lamellatus and H.sellatus.The results of PCR detection displayed that the carrier rate of JWB phytoplasma was 13.33%,12.67%and 10.00%in samples of H.lamellatus,H.sellatus and L.striatellus,respectively.No JWB phytoplasma was detected in other insect samples.The JWB phytoplasma was detected for the first time in L.striatellus,indicating that L.striatellus may be a potential vector insect for JWB.Accordingly,the monitoring and control of vector insects are conducive to the management of JWB.The study aims to provide reference for the control of vector insects to block the transmission chain of JWB.
关键词
枣疯病/叶蝉/植原体/介体昆虫/种群消长动态/陕西关中地区Key words
jujube witches'broom/leafhopper/phytoplasma/vector insects/population dynamics/Guanzhong area of Shaanxi province引用本文复制引用
出版年
2024
NETL
NSTL
万方数据