Transcriptome Analysis of Head Kidney Anti-LMBV Responses in Largemouth Bass(Micropterus salmoides)
The transcription profile of Micropterus salmoides against LMBV infection was analyzed to reveal the immune response mechanism of Micropterus salmoides in response to LMBV infection.The head kidney tissues of largemouth bass infected with LMBV 72h were analyzed by transcriptome sequencing using Illumina NovaSeq 6000 sequencing platform.A total of 5953 differentially expressed genes(DEGs)were obtained,of which 1704 were up-regulated and 4249 were down-regulated.Through GO function annotation,DEGs were mainly related to single-organism process,metabolic process,membrane,cell,binding,catalytic activity,etc.The KEGG enrichment analysis showed that DEGs were mainly enriched in metabolic pathways such as glycolysis,primary bile acid biosynthesis.There were 4 immune response-related signaling pathways enriched including cytokine-cytokine receptor interaction,ECM-receptor interaction,phagosome and intestinal immune network for IgA production.The immune-related increased DEGs were cxcr4,il10,mrc,ncf4,itgb2,il27,ccl25,etc.while the decreased degs were cxcl12,tgfb3,il20ra,col4a5,itgb1,etc.PPI analysis showed that itgb1,itga8 and itgb6 were the hub genes of immune-related DEGs.This study analyzed the transcription profile of largemouth bass infected with LMBV to provide a theoretical basis for the molecular mechanism and disease prevention of largemouth bass immunity against LMBV.
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