In order to investigate the impact of Trichoderma-enriched bio-organic fertilizer on the yield,quality and soil nutrient availability in tomatoes cultivated in costal saline soil,the salt tolerance and mycelial morphology of two Trichoderma spp.were examined using plate growth.A field experiment was conducted in Ningbo coastal saline soil,with five treatment groups:no fertilizer(CK),chemical fertilizer at a rate of 2200 kg/hm2(T1),bio-organic fertilizer at a rate of 9 t/hm2(T2),bio-organic fertilizer at a rate of 18 t/hm2(T3),and bio-organic fertilizer combined with chemical fertilizer at rates of 6 t/hm2+1500 kg/hm2(T4),respectively.The bio-organic fertilizer was prepared using T.asperellum W1 strain.Lab experiment indicated that T.asperellum W1 exhibited superior growth ability compared to Trichoderma LX-132,thus validating its suitability for further testing.Based on phylogenetic tree analysis and mycelial morphology,W1 was provisionally identified as T.asperellum.Field experiment demonstrated that the application of bio-organic fertilizer resulted in higher organic matter content compared to non-bio-organic fertilizer treatments.Specifically,T3 showed an increase in organic matter content by 16.5％and 10.7％when compared with CK and T1,respectively.The bio-organic fertilizer treatment also led to improved quality and higher yields,with T3 having the highest Vc content and sugar-acid ratio.These values increased by 5.39％and 6.18％compared to CK,and by 2.35％and 4.71％compared to T1,respectively.The combination of bio-organic and chemical fertilizer(T4)exhibited the most significant yield-increasing effect,with T4 showing an increase of 117％and 6.26％compared to CK and T1,respectively.The results of this research provide technical support and a theoretical foundation for the development of new bio-organic fertilizers.
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